Use the Invisorb® Spin Virus RNA Mini Kit for reliable isolation of high-quality RNA from viruses found in a diverse range of starting materials. The kit simplifies viral RNA isolation by combining efficient lysis of the starting material and the inactivation of exogenous and endogenous RNases to prevent degradation.
- RT-PCR templates in 25 minutes with high sensitivity at low virus titers
- Safe handling of infectious samples by using capped spin columns
- For In Vitro Diagnostic Use (CE-IVD)*
Starting Material Average Yield Preparation Time up to 200 µl serum, plasma, cell-free body fluids, rinse liquid from swabs cell culture supernatants
up to 50 µl whole blood
1 x 106 mammalian cells
max. 20 mg tissue
depending on viral titer 25 min
The sample is lysed in an optimized lysis buffer and proteins are degraded during the lyses with Proteinase K. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. The addition of Carrier RNA is necessary for the enhancement of DNA recovery in samples with low virus titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The liberated RNA is bound onto the membrane of the Spin Filter. Contaminants are removed by repeated washing steps and the purified viral RNA can be eluted in a small volume of RNase-free water. Due to the high purity, the isolated viral RNA is ready to use for a broad panel of downstream applications like:
- Taq Man analysis and array technologies
- RNA dot blots
- cDNA transcription
- in vitro translation
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
1. Sensitive detection of Norwalk Virus from stool samples
Viral RNA was isolated from 50 mg NLV infected fecal samples using the Invisorb Spin Virus RNA Mini Kit. The extracted viral RNA was amplified on a Rotor-Gene™ 3000 using a NLV specific PCR. 10 µl of eluted NLV sample RNA was used per cDNA synthesis.
Data kindly provided by C. Helmeke, H-M., Irmscher, State Office of Consumer Protection, Saxony-Anhalt, Department Medical Microbiology, Magdeburg, Germany
User manual download
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|Product||Package size||Catalogue No|
|Invisorb Spin Virus RNA Mini Kit||250 purifications||1040300300|